Add Embryos to Inventory or Transferred Embryos
3 min
pulse allows you to add fresh or frozen embryos to inventory to streamline embryo transfer tasks while out on the farm and using the mobile app use the batch import feature to quickly add multiple embryos, such as those harvested from an , or add individual embryos or multiple identical embryos as needed you can also add transferred embryos directly to the transferred embryos docid\ kr1cg8jwjcxb6rvl8zqto page using the same batch import process and file you can find this feature under the dairycomp/mydc docid\ ezfejzdkbcfov b69aupr > embryos docid\ ezfejzdkbcfov b69aupr menu the options to add embryos are available from the menu or the embryo management tabs before you add embryos to inventory prior to entering your embryos to inventory, be sure to have all required details verify that the lab report or other listing that accompanied the delivery of your embryos is handy and available for reference note that the following details are required to add embryos to inventory code or donor id, name, or iso id fertilization date frozen date (for frozen embryos) in addition to the required items, other information that is useful when preparing to add embryos includes embryo breed straw or mating details embryo counts embryo requirements for transfer pulse limits the transfer of embryos to those with the highest potential for success embryos must meet the following requirements to qualify for transfer embryo transfers are typically done with 4 to 8 day old embryos in pulse, you can only enter data for embryos that are less than 12 days old ( < 12) as noted in enter completed transfers as soon as possible! docid\ xe24w8so0ee7cfvfn3xeb , you must be prompt with your data entry to capture embryo transfers in pulse must be grade 1 3 1 good 2 fair 3 poor must be stage 4 9 4 morula 5 early blastocyst 6 blastocyst 7 expanded blastocyst 8 hatched blastocyst 9 expanded and hatched blastocyst expand the sections below to learn more import embryos in batch follow the steps below to continue download the template and upload data select import embryos in batch from the menu bar or the options button click download the template to save the template file use the template to enter your embryo data, noting the required fields ( bolded in the template) donor embryo breed fertilization date recovery type straw number be sure to also add the following for frozen embryos frozen date (required for frozen embryos) freeze method (required for frozen embryos) important if you are not adding data for optional fields, leave them empty make sure the file extension remains xlsx if you change the extension, the file upload will fail, resulting in an "invalid file type" error embryo details the bull whose semen was used to fertilize the donor's egg to produce the embryo if this column shows pool , it means that an was fertilized by from a pool containing the semen of multiple sires donor tthe female who provided the embryo or oocyte (sometimes referred to as the donor dam) embryo breed the embryo breed select from the standard list of naab breeds fertilization date date the donor egg was fertilized using the sire semen recovery type the recovery type used flush is used for embryos ivf for in vitro fertilization ( ) other for neither of the above tank the liquid nitrogen storage tank containing the cane with the embryo's straw incubator the warming unit containing the embryo's straw or mating cane the container holding the embryo's straw mating a description of the embryo mating details, such as sire, donor, and fertilization date may contain multiple embryos straw number number of the straw holding the embryo canes contain multiple straws and each straw holds a single embryo stage the stage at which the embryo was harvested 1 unfertilized 2 simple celled 3 early morula 4 morula 5 early blastocyst 6 blastocyst 7 expanded blastocyst 8 hatched blastocyst 9 expanded and hatched blastocyst grade the quality of the embryo 1 good 2 fair 3 poor 4 dead (or degenerating) lab id identifier for the lab from which the embryo was purchased lab embryo id identifier for the embryo from the lab this value differs from the pulse embryo id embryo owner name or company that owns the embryo frozen date date the embryo was frozen freeze method the method used to freeze the embryo direct transfer (dt) is the process of using high levels of cryoprotectant to quickly cool an embryo to freezing a common cryoprotectant used for this process is ethylene glycol slow is the process of using low levels of cryoprotectant and a programmable freezing machine to slowly lower the temperature of the embryo to freezing recipient the animal that received the embryo transfer including the recipient and transfer details automatically adds a transfer event to the recipient animal see import transferred embryos docid\ xe24w8so0ee7cfvfn3xeb for more information transferred the embryo technician who completed the transfer transferred date date the embryo was transferred to the recipient breeding code the code associated with the breeding activity check your dairy's breeding codes docid\ vlm0f3i4dqdhwhbpuowes for more details on each code drag and drop the completed template file into the upload box, or click browse to navigate to your file select the date format click continue to preview and confirm the data preview data and confirm platform provides a summary and preview of the embryo data being imported hover over any error/warning icons to display the error message error warns that a required detail is missing or if the data included is not within the expected range warning indicates an issue with the data, such as a straw number that is already in use for a frozen embryo if necessary, return to your embryo batch file, resolve any errors, and upload the file again when ready, click import to import your embryos import summary after your embryo data is imported, you can download a list of the embryos that were not included you can use this list to identify and resolve any errors, and if necessary, upload the updated file import transferred embryos in addition to importing embryos to inventory, you can use the same template file to import completed transfers follow the import embryos in batch docid\ xe24w8so0ee7cfvfn3xeb steps and be sure to include the required details in your template file recipient the animal that received the embryo transfer transferred date date the embryo was transferred to the recipient you can also include optional details if your dairy tracks them transferred tech the embryo technician who completed the transfer breeding code the code associated with the breeding activity check your dairy's breeding codes docid\ vlm0f3i4dqdhwhbpuowes for more details on each code after the import, the embryo is added directly to the transferred embryos docid\ kr1cg8jwjcxb6rvl8zqto tab pulse also automatically adds a transfer event to the recipient's cowcard for the transferred date you specified add one embryo or multiple embryos with same details select add embryo from the menu bar or the options button complete all required fields and any additional details for the embryo note to add multiple embryos with the same details, use the number of embryos/straws field for fresh embryos and the from straw / to straw fields for frozen embryos pulse will calculate the number of frozen embryos you're adding based on the difference between the straw numbers embryo details the bull whose semen was used to fertilize the donor's egg to produce the embryo if this column shows pool , it means that an oocyte was fertilized by ivf from a pool containing the semen of multiple sires donor tthe female who provided the embryo or oocyte (sometimes referred to as the donor dam) embryo breed the embryo breed select from the standard list of naab breeds fertilization date date the donor egg was fertilized using the sire semen recovery type the recovery type used flush is used for embryos ivf for in vitro fertilization ( ) other for neither of the above tank the liquid nitrogen storage tank containing the cane with the embryo's straw incubator the warming unit containing the embryo's straw or mating cane the container holding the embryo's straw mating a description of the embryo mating details, such as sire, donor, and fertilization date may contain multiple embryos straw number number of the straw holding the embryo canes contain multiple straws and each straw holds a single embryo from straw the first straw number that contains this embryo to straw the last straw number that contains this embryo this allows pulse to calculate the total number of embryos to be added for instance, if you add embryos from straw 6 to straw 11, a total of 6 embryos are added note to add a single embryo, do not include from straw and to straw values stage the stage at which the embryo was harvested 1 unfertilized 2 simple celled 3 early morula 4 morula 5 early blastocyst 6 blastocyst 7 expanded blastocyst 8 hatched blastocyst 9 expanded and hatched blastocyst grade the quality of the embryo 1 good 2 fair 3 poor 4 dead (or degenerating) lab id identifier for the lab from which the embryo was purchased lab embryo id identifier for the embryo from the lab this value differs from the pulse embryo id embryo owner name or company that owns the embryo frozen date date the embryo was frozen freeze method the method used to freeze the embryo direct transfer (dt) is the process of using high levels of cryoprotectant to quickly cool an embryo to freezing a common cryoprotectant used for this process is ethylene glycol slow is the process of using low levels of cryoprotectant and a programmable freezing machine to slowly lower the temperature of the embryo to freezing click add if you made a mistake or need to change embryo details, see edit or delete embryos docid\ fbeckbxg3aawwhpxvadtu